Strep epitope Tag antibody [HL1251]

Cat. No. GTX636653

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1251
Isotype	IgG
Application	WB, ICC/IF, IP, IHC-P (cell pellet)
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX636653 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IP	Assay dependent
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Strep epitope Tag

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910030

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636653 ICC/IF Image

Strep epitope Tag antibody [HL1251] detects Strep epitope Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1251] (GTX636653) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636653 IHC-P (cell pellet) Image

Strep epitope Tag antibody [HL1251] detects Strep epitope Tag protein at cytoplasm by immunohistochemical analysis.
Sample:Paraffin-embedded mock and Strep Tagged protein transfected 293T cell pellet.
Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1251] (GTX636653) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636653 IP Image

Immunoprecipitation of Strep epitope Tag protein from 293T whole cell extracts using 5 μg of Strep epitope Tag antibody [HL1251] (GTX636653).
Western blot analysis was performed using Strep epitope Tag antibody [HL1251] (GTX636653).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

GTX636653 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [HL1251] (GTX636653) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Strep epitope Tag antibody [HL1251]

Cat. No. GTX636653

Host

Clonality

Clone Name

Isotype

Application

Reactivity